Plasmid
Production Platform
Plasmid
Production
Platform
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Our plasmid platform offers high-quality plasmids for various gene therapy applications. We utilize advanced manufacturing technology to ensure purity, consistency, and reliability of our products. Our plasmids are designed for specific gene therapies like viral vectors, CRISPR/Cas9 gene editing, and RNA interference, and can be used with different delivery methods including electroporation, lipofection, and viral transduction.


Quality control measures are implemented at every stage of manufacturing to maintain the highest standards. Detailed documentation is provided for each product.


Trusted by renowned gene therapy researchers and pharmaceutical companies worldwide, our plasmid platform enables the development of innovative gene therapies. With our high-quality plasmids and technical support, we aim to enhance patient lives.

Why Choose Us

  • 24/7 Facilities with Extremely Short Lead Times
  • Reduction of Manufacturing Costs (Labor & Materials)
  • High-Quality Plasmids Meet the Demands of Various Applications
  • Automatic Fill and Finish in cGMP Suites
  • Capability to Develop a Lyophilization Cycle and Scale up to GMP Manufacturing

Microbial Cell Banking

  • 1
    Plasmid Construction
  • 2
    DNA Transformation
  • 3
    Screening Bacterial Colonies
  • 4
    Bacterial Research Cell
    Bank (RCB) Generation
  • 5
    Bacterial Master Cell
    Bank (MCB) Generation
  • 6
    Bacterial Working Cell
    Bank (WCB) Generation

Microbial Bank Analysis Methods

Microbial Bank
Analysis Methods

No.AssayMethod
01ViabilityPlate-Count Method
02Strain IdentityGram Stain Analysis
03Biochemical IDBiochemical Confirmatory Test
0416S rDNA SequenceSequencing
05Microbial PurityPlate Method
06Antibiotic ResistancePlate Method
07Restriction Enzyme MappingAgarose Gel Electrophoresis (AGE)
08Plasmid RetentionPlate Method
09Plasmid Copy NumberqPCR
10Sequence IdentitySequencing
11Phage ContaminationDouble-Layer Agar (DLA)
12MorphologyTransmission Electron Microscope (TEM)
13Whole Genome Sequencing (WGS)Sequencing

GMP Plasmid
Manufacturing Process

  • 1
    MCB/WCB
    Seed Recovery
  • 2
    Seed Culture
  • 3
    Upstream Fermentation

    Up to 30L single-use fermentor

  • 4
    Bacteria Lysis
  • 5
    Downstream Purification

    2-step chromatography

  • 6
    Sterile Fill and Finish
  • 7
    Quality Control
    & Release Testing
  • 8
    Documentation and
    Products Delivery

Plasmid Analysis Methods and Specifications

Plasmid Analysis
Methods and
Specifications

No.AssayMethodSpecificationIndustrial
Grade		GMP
Grade
01AppearanceVisual InspectionClear and Colorless Solution√√
02ConcentrationUltraviolet Spectroscopy (UV)1.0±0.2 mg/mL√√
03pHUSP<791>6.5–8.5√√
04IdentityRestriction MapIdentical to Theoretical or Identical to Reference Map√√
05Purity A260/A280Ultraviolet Spectroscopy (UV)1.8–2.0√√
06% SupercoiledHPLC≥85%√√
07Residual Host ProteinELISA≤0.5%√√
08Residual Host Genomic DNAqPCR≤0.5%√√
09Residual Host RNART-qPCR≤0.5%√√
10Bacterial EndotoxinUSP<85><10EU/mg√√
11SterilityUSP<71>No Growth-√
12Sequence IdentitySequencingIdentical to Theoretical Sequence-√

Plasmid Linearization

Why Choose Us

High Yield

High Purity

  • 1
    High Quality Supercoiled Plasmid
  • 2
    Restriction Enzymatic Digestion
  • 3
    Chromatography
  • 4
    Ultrafiltration & Diafiltration
  • 5
    Quality Control

Start Bioservices currently provides a selection of proprietary off-the-shelf plasmids designed to expedite cell and gene therapy programs. These AAV Helper plasmids are available in both industrial grade and GMP grade, allowing us to better meet the diverse requirements of our clients.

Off-the-shelf Plasmid Catalog

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Product TypeProduct DescriptionCatalog No.List Price (USD)Resistance Gene
AAV Helper
Plasmid		AAV Helper Plasmid
– Industrial Grade		Sbio-AAV-HPIG-8mg$344Kanamycin
Sbio-AAV-HPIG-20mg$860
Sbio-AAV-HPIG-40mg$1,280
Sbio-AAV-HPIG-60mg$1,920
Sbio-AAV-HPIG-80mg$2,560
Sbio-AAV-HPIG-100mg$3,200
AAV Helper Plasmid
– GMP Grade		Sbio-AAV-HPGG-100mg$9,450
Sbio-AAV-HPGG-500mg$45,500
Sbio-AAV-HPGG-1g$89,500

Customized Plasmid Catalog

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Product TypeGuaranteed AmountEach Lot (1st time)Subsequent Each Lot
Industrial Grade>1g$13,000$9,800
>2g$16,000$12,600
GMP Grade>1g$64,000$48,000
>2g$80,000$60,000
>5g$140,000$105,000